Cysteine-2 and Cys30 are essential for chlorophyll-binding activity of the water-soluble chlorophyll-binding protein (WSCP) of Chenopodium album
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چکیده
منابع مشابه
Structural mechanism and photoprotective function of water-soluble chlorophyll-binding protein.
A water-soluble chlorophyll-binding protein (WSCP) is the single known instance of a putative chlorophyll (Chl) carrier in green plants. Recently the photoprotective function of WSCP has been demonstrated by EPR measurements; the light-induced singlet-oxygen formation of Chl in the WSCP tetramer is about four times lower than that of unbound Chl. This paper describes the crystal structure of th...
متن کاملIntegration of a chlorophyll-binding protein into Escherichia coli membranes in the absence of chlorophyll.
The mechanism by which a protein integrates posttranslationally into a membrane can involve the composition of the membrane itself, domains within the inserting polypeptide, and a number of associating proteins. Some integral membrane proteins do not accumulate to normal levels when certain pigments are deficient, and this has been interpreted to mean that such proteins may be rapidly degraded ...
متن کاملSynthesis and DNA-binding properties of water-soluble cationic pyropheophorbides derived from chlorophyll a/b.
A series of mono-, di-, tetra- and hexacationic esters of pyropheophorbide a/b have been designed and synthesized to explore the intercalation of their phorbine ring between the base pairs of double-helical DNA and the influence of their peripheral substituents on the DNA interactions. Mono-(1), di-(2, 3) and tetra-(4, 5) cationic pyropheophorbides are soluble as an oligomeric aggregate in HEPE...
متن کاملMolecular Cloning and Functional Expression of A Class IIA Water-Soluble Chlorophyll-Binding Protein From Brassica nigra
Non-photoconvertible water-soluble chlorophyll-binding proteins, called Class II WSCPs, have been identified in some Brassicaceae plants. Although the WSCP in black mustard (Brassica nigra) was the second Class II WSCP to be reported by Murata and Murata, the cDNA has not been cloned until now. In this study, we cloned a cDNA encoding the black mustard WSCP (BmWSCP) and analyzed its chlorophyll...
متن کاملCharacterization of chlorophyll binding to LIL3
The light harvesting like protein 3 (LIL 3) from higher plants, has been linked to functions in chlorophyll and tocopherol biosynthesis, photo-protection and chlorophyll transfer. However, the binding of chlorophyll to LIL3 is unclear. We present a reconstitution protocol for chlorophyll binding to LIL3 in DDM micelles. It is shown in the absence of lipids and carotenoids that reconstitution of...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 2014
ISSN: 0916-8451,1347-6947
DOI: 10.1080/09168451.2014.940274